King Agaricus 100 for Pets

In healthy subjects, after oral intake of 9 g of open-pollinated Agaricus KA21 per day (3 to 22 times the standard intake) for 6 months, the product's safety was confirmed with no side effects and no effect on liver and kidney function values.
Source: https://pubmed.ncbi.nlm.nih.gov/18604247/

The safety of open-pollinated Agaricus KA21 was confirmed in a test tube study and an animal study in which 1 g per 1 kg of body weight (approximately 16 to 125 times the standard intake) was ingested.　
Source: https://pubmed.ncbi.nlm.nih.gov/22506574/

Natural killer cells, a type of immune cell, were activated in healthy subjects by taking 3 g of open-pollinated Agaricus KA21 per day for one week. *Natural killer cells are immune cells that attack cancer cells and pathogens that cause infections.
Source: https://pubmed.ncbi.nlm.nih.gov/18604247/

Based on the above research results, intake of Agaricus KA21 is thought to enhance immunity and prevent infection.

Mice receiving the anti-cancer drug (5-FU) were fed orally with feed containing open-pollinated Agaricus KA21 and were evaluated for leukopenia, diarrhea, weight loss, feeding disorder, renal disorder, gastrointestinal disorder, and hair loss, which are side effects of the anti-cancer drug.

As a result, the ingestion of open-pollinated Agaricus was confirmed to reduce the side effects of anti-cancer drugs, but not that of house-grown Agaricus. The impact of KA21 on reducing the side effects of anti-cancer drugs may be helpful for the physical condition of cancer patients. The potent antioxidant activity of open-pollinated Agaricus may have reduced the side effects of anti-cancer drugs.
Source: https://pubmed.ncbi.nlm.nih.gov/32463996/

When wound model mice were fed orally with feed containing open-pollinated Agaricus KA21, the production of skin collagen type 3 was stimulated, and its wound-healing promoting effect was confirmed. The skin moisturizing effect against dryness was also confirmed in the same study.　
* Presented at the 39th Annual Meeting of the Japanese Society of Aesthetic Dermatology, paper in preparation for submission

When healthy people orally ingested 900 to 1500 mg of open-pollinated Agaricus KA21 per day, the effects of reduced fatigue, better wakefulness (improvement of sleep quality), reduction of stiff shoulders, and improvement of sensitivity to cold were confirmed after 6 and 12 weeks of ingestion. In addition, the effects of improving hair loss and graying were confirmed after 6 weeks and 12 weeks of intake of 1500 mg/day.
Source: https://pubmed.ncbi.nlm.nih.gov/26756293/

When mice modeling hair loss were orally fed diets containing open-pollinated Agaricus KA21 and house-cultivated Agaricus KA21, the effect of hair growth was confirmed in the open-pollinated Agaricus KA21 intake group. The results were presented at the 10th International Medicinal Mushrooms Conference. Paper in preparation for submission.

When mice with liver inflammation caused by intraperitoneal administration of carbon tetrachloride were orally given feed containing either open-pollinated Agaricus KA21 or house-grown Agaricus KA21, only the group fed with open-pollinated Agaricus KA21 showed a protective effect on the liver.
Source: https://pubmed.ncbi.nlm.nih.gov/25418207/

When fatty liver model mice with a high-fat diet were given feed containing open-pollinated Agaricus KA21, the antioxidant and anti-inflammatory effects of Agaricus KA21 inhibited liver fibrosis caused by NASH. Intake of open-pollinated Agaricus KA21 may be effective in preventing NASH.
Source: https://pubmed.ncbi.nlm.nih.gov/31689883/

Hypertensive model rats were fed a diet containing open-pollinated Agaricus KA21, which reduced heart rate and blood pressure, protected the heart, and improved circulatory function.
Source: https://pubmed.ncbi.nlm.nih.gov/24433071/

Healthy people were given 3g of open-pollinated Agaricus KA21 per day for 3 months, which resulted in reduced body weight, lower body fat percentage, decreased visceral fat mass, cholesterol level, and blood glucose level.　
Source 1: https://pubmed.ncbi.nlm.nih.gov/18604247/

Akkermansia muciniphila, a type of intestinal bacteria, effectively improves obesity and diabetes.　
Source 2: https://pubmed.ncbi.nlm.nih.gov/27892954/
Source 3: https://pubmed.ncbi.nlm.nih.gov/23671105/

* Ingestion of open-pollinated Agaricus KA21 in a mouse study showed a proliferative effect of Bacillus subtilis Ackermansia muscinifera. Japanese patent pending.

When healthy people were fed 3 g of open-pollinated Agaricus KA21 per day for 3 months, body weight, body fat percentage, visceral fat mass, cholesterol level, and blood glucose level were reduced.　
Source: https://pubmed.ncbi.nlm.nih.gov/18604247/

Oral feeding of diabetic model mice with feed containing open-pollinated Agaricus KA21 improved blood glucose levels.
Source: https://pubmed.ncbi.nlm.nih.gov/21562638 /

Akkermansia muciniphila, a type of intestinal bacteria, effectively improves obesity and diabetes.　
Source 1: https://pubmed.ncbi.nlm.nih.gov/27892954/
Source 2: https://pubmed.ncbi.nlm.nih.gov/23671105/

* Ingestion of open-pollinated Agaricus KA21 in a mouse study showed a proliferative effect of Bacillus subtilis Ackermansia muscinifera. Japanese patent pending.

When healthy people orally ingested 900 to 1500 mg of open-pollinated Agaricus KA21 per day, the effects of reduced fatigue, better awakening (improved sleep quality), reduced stiff shoulders, and improved sensitivity to cold were confirmed after 6 and 12 weeks of ingestion. In addition, when 1500 mg was taken per day, improvement of hair loss and graying was confirmed after 6 weeks and 12 weeks.
Source: https://pubmed.ncbi.nlm.nih.gov/26756293/

When wound model mice were fed orally with feed containing open-pollinated Agaricus KA21, the production of collagen type 3 was stimulated, confirming its wound-healing promoting effect. The skin moisturizing effect against dryness was also confirmed in the same study.
* Presented at the 39th Annual Meeting of the Japanese Society of Aesthetic Dermatology. Paper in preparation for submission.

Ingestion of open-pollinated Agaricus KA21 in mice confirmed the proliferative effect of Ackermansia muscinifera, a type of intestinal bacteria. Akkermansia muciniphila is effective in improving obesity and diabetes. Japanese patent pending.
Source 1: https://pubmed.ncbi.nlm.nih.gov/27892954/
Source 2: https://pubmed.ncbi.nlm.nih.gov/23671105/

Akkermansia muciniphila affects the efficacy of immune checkpoint inhibitors.　
Source: https://pubmed.ncbi.nlm.nih.gov/29097494/